概要: Pregnant women appear to be at increased risk for severe outcomes associated with COVID-19, but the pathophysiology underlying this increased morbidity and its potential impact on the developing fetus is not well understood. In this study of pregnant women with and without COVID-19, we assessed viral and immune dynamics at the placenta during maternal SARS-CoV-2 infection. Viral RNA was only rarely detected in the placentas from SARS-CoV-2-positive women in our cohort, with only 1/11 positive for infection at the maternal-fetal interface. Through bulk RNA transcriptomic analyses, we found that placentas from SARS-CoV-2-positive pregnancies exhibited inflammatory markers of immune activation, even in the majority of samples which did not show local invasion of the virus. These markers are associated with pregnancy complications such as preeclampsia and poor fetal outcomes. Overall, this study suggests that SARS-CoV-2 is associated with immune activation at the maternal-fetal interface even in the absence of detectable local viral invasion. While this likely represents a protective mechanism shielding the placenta from infection, inflammatory changes in the placenta may also contribute to poor pregnancy outcomes and thus warrant further investigation.
项目整体设计: mRNA profiles of placental villous tissue at term delivery from control and SARS-CoV-2-positive mothers
Villi were dissected from fresh placental tissue and rinsed three times in PBS. Villous samples were homogenized and centrifuged before RNA isolation using the Qiagen RNAeasy Mini Kit.
建库方案:
RNA libraries were prepared for sequencing following rRNA depletion using standard Illumina protocols
测序信息
分子类型:
rRNA- RNA
库的片段类型:
PAIRED
库的链类型:
-; Forward
测序平台:
ILLUMINA
测序仪型号:
Illumina HiSeq 2500
链特异性:
Unspecific; Specific
样本
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组织
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细胞亚型
细胞系
疾病
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发育阶段
突变/变异
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Condition Detail
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平均测序片段长度_1
平均测序片段长度_2
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文章
An Immuno-Cardiac Model for Macrophage-Mediated Inflammation in COVID-19 Hearts.
Circulation research . 2021-04-15 [PMID:
33853355]
Maternal respiratory SARS-CoV-2 infection in pregnancy is associated with a robust inflammatory response at the maternal-fetal interface.
Med (New York, N.Y.) . 2021-04-30 [PMID:
33969332]
