生长方案:	-
处理方案:	-
提取方案:	RNA from the different cell lines was extracted using 500 µl TRIzol Reagent (Ambion) directly on 10-cm dishes (cell confluency ∼80%, ∼1,000,000 cells). Lysed cells in TRIzol were scraped into tubes, extracted twice with chloroform, the RNA was precipitated with 0.7 volume isopropanol after addition of 20 µg glycogen, and resuspended in UltraPure distilled water. RNA concentration and quality were determined by Nanodrop (ratio 260/230 and 260/280 above 1.8) and Agilent 2100 BioAnalyzer (RIN above 8). Total RNA was used for northern blot analysis, RiboMethSeq, and RT-PCR and RNase H analysis.
建库方案:	For deep sequencing, total RNA was prepared from 3 separate dishes for each sample and shipped to Novogene Corporation Inc. (Sacramento, CA) for cDNA library preparation (250~300 bp inserts) and Illumina sequencing (PE150). The RNA was prepared and sequenced one year apart in the USA and in China in two batches, one for the P1, KD1a, and KD1b cells and one for the P2 and KD2 cells.

分子类型:	total RNA
库的片段类型:	PAIRED
库的链类型:	-
测序平台:	ILLUMINA
测序仪型号:	Illumina HiSeq 2000
链特异性:	Unspecific

数据来源	GEN样本编号	GEN数据集编号	系列编号	项目编号	样本编号	样本名称	生物样本编号	样本访问号	实验访问号	释放时间	提交时间	最后更新时间	物种	种族	族裔	年龄	年龄单位	性别	来源名称	组织	细胞类型	细胞亚型	细胞系	疾病	疾病状态	发育阶段	突变/变异	表型	Condition Detail	生长方案	处理方案	提取方案	建库方案	分子类型	库的片段类型	链特异性	库的链类型	加标（Spike-In）	测序方法	测序平台	测序仪型号	细胞数	测序片段数	碱基数	平均测序片段长度_1	平均测序片段长度_2	唯一比对率	多重比对率	覆盖度