概要: SARS-CoV-2 infection causes a wide spectrum of clinical manifestations in human, and olfactory dysfunction represents as one of the most predictive and common symptoms in COVID-19 patients. Herein we demonstrate intranasal inoculation of SARS-CoV-2 induces robust viral replication in the olfactory epithelium (OE), not olfactory bulb (OB), resulting in transient olfactory dysfunction in humanized ACE2 mice. To decipher the underlying mechanism of the observed olfactory dysfunction in SARS-CoV-2 infected mice at the molecular level, RNA-Seq analyses of the OE and OB samples from SARS-CoV-2 infected mice were performed in comparison with that from the control animals
项目整体设计: To decipher the underlying mechanism of the observed olfactory dysfunction in SARS-CoV-2 infected mice at the molecular level, RNA-Seq analyses of the OE and OB samples from SARS-CoV-2 infected mice (2 and 4 dpi) were performed in comparison with that from the control animals.
Total RNA from lung were extracted using TRIzol (Invitrogen, Carlsbad, CA, USA) and DNase I (NEB, USA) treated, respectively.
建库方案:
Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (#E7530L, NEB, USA) following the manufacturer’s recommendations
测序信息
分子类型:
polyA(+) RNA
库的片段类型:
PAIRED
库的链类型:
-
测序平台:
ILLUMINA
测序仪型号:
Illumina NovaSeq 6000
链特异性:
unspecific
样本
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疾病
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文章
SARS-CoV-2 infection in the mouse olfactory system.
