PRJNA734335: High-fat high-sugar diet-induced changes in the lipid metabolism are associated with mildly increased COVID-19 severity and delayed recovery in the Syrian hamster
概要: Pre-existing comorbidities such as obesity or metabolic diseases can adversely affect the clinical outcome of COVID-19. Chronic metabolic disorders are globally on the rise and often a consequence of an unhealthy diet, referred to as a Western Diet. For the first time in the Syrian hamster model, we demonstrate the detrimental impact of a continuous high-fat high-sugar diet on COVID-19 outcome. We observed increased weight loss and lung pathology, such as exudate, vasculitis, hemorrhage, fibrin, and edema, delayed viral clearance and functional lung recovery, and prolonged viral shedding. This was accompanied by and increased trend of systemic IL-10 and IL-6, as well as a dysregulated serum lipid response dominated by polyunsaturated fatty acid-containing phosphatidylethanolamine, recapitulating cytokine and lipid responses associated with severe human COVID-19. Our data support the hamster model for testing restrictive or targeted diets and immunomodulatory therapies to mediate the adverse effects of metabolic disease on COVID-19.
项目整体设计: High-fat high-sugar diet vs. Regular diet
| 生长方案: |
- |
| 处理方案: |
Four to six-week-old male Syrian Golden hamsters (ENVIGO) were randomly assigned to either regular rodent chow (Teklad Global 16% Protein Rodent Diet, Envigo) or a HFHS diet for 16 weeks (Purina Chow #5001 with 11.5% Corn Oil, 11.5% Coconut Oil, 0.5% Cholesterol, 0.25% Deoxycholic Acid, and 10% Fructose: Dyets Inc., Dyet#615088) |
| 提取方案: |
RNA was collected from each sample and passed through Qiashredder column (Qiagen) at 21,000 x g for 2 min to homogenize any remaining genomic DNA in the aqueous phase. Aqueous phase was combined with 600 µL of RLT lysis buffer (Qiagen, Valencia, CA) with 1% beta mercaptoethanol (MilliporeSigma) and RNA was extracted using Qiagen AllPrep DNA/RNA 96-well system. |
| 建库方案: |
One hundred nanograms of RNA was used as input for rRNA depletion and NGS library preparation following the Illumina Stranded Total RNA Prep Ligation with Ribo-Zero Plus workflow (Illumina) |
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rRNA- RNA |
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PAIRED |
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Forward |
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ILLUMINA |
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Illumina NextSeq 500 |
| 链特异性: |
Specific |
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High-Fat High-Sugar Diet-Induced Changes in the Lipid Metabolism Are Associated with Mildly Increased COVID-19 Severity and Delayed Recovery in the Syrian Hamster.
Viruses . 2021-12-14 [PMID:
34960775]
