Gene Expression Nebulas
基于标准化流程分析的转录图谱综合数据库

Gene Expression Nebulas

多物种转录图谱整合数据库

PRJNA744246: Next Generation Sequencing of Wild Type and OsMIR393b Transcriptomes

来源: NCBI / GSE179594
提交时间: Jul 06, 2021
释放时间: Dec 21, 2022
最后更新时间: May 12, 2023

概要: Acquiring the gene network required for somatic embryogenesis by comparing the differentially expressed genes.

项目整体设计: mRNA profiles of mature embryos of wild type (NIP) and OsMIR393b (OEB)

GEN 数据集:
GEND000522
测序方法:
物种:
组织:
健康状况:
细胞类型:
细胞系:
发育阶段:
方案
生长方案: The plants were grown in greenhouse at 16 h light, 28 ℃/8 h dark, 24 ℃ cycle; The plants were grown in greenhouse at 16 h light, 28 ℃/8 h dark, 25 ℃ cycle; The plants were grown in greenhouse at 16 h light, 28 ℃/8 h dark, 26 ℃ cycle; The plants were grown in greenhouse at 16 h light, 28 ℃/8 h dark, 27 ℃ cycle; The plants were grown in greenhouse at 16 h light, 28 ℃/8 h dark, 28 ℃ cycle; The plants were grown in greenhouse at 16 h light, 28 ℃/8 h dark, 29 ℃ cycle; The plants were grown in greenhouse at 16 h light, 28 ℃/8 h dark, 30 ℃ cycle; The plants were grown in greenhouse at 16 h light, 28 ℃/8 h dark, 31 ℃ cycle; The plants were grown in greenhouse at 16 h light, 28 ℃/8 h dark, 32 ℃ cycle; The plants were grown in greenhouse at 16 h light, 28 ℃/8 h dark, 33 ℃ cycle; The plants were grown in greenhouse at 16 h light, 28 ℃/8 h dark, 34 ℃ cycle; The plants were grown in greenhouse at 16 h light, 28 ℃/8 h dark, 35 ℃ cycle; The plants were grown in greenhouse at 16 h light, 28 ℃/8 h dark, 36 ℃ cycle; The plants were grown in greenhouse at 16 h light, 28 ℃/8 h dark, 37 ℃ cycle; The plants were grown in greenhouse at 16 h light, 28 ℃/8 h dark, 38 ℃ cycle; The plants were grown in greenhouse at 16 h light, 28 ℃/8 h dark, 39 ℃ cycle; The plants were grown in greenhouse at 16 h light, 28 ℃/8 h dark, 40 ℃ cycle; The plants were grown in greenhouse at 16 h light, 28 ℃/8 h dark, 41 ℃ cycle; The plants were grown in greenhouse at 16 h light, 28 ℃/8 h dark, 42 ℃ cycle; The plants were grown in greenhouse at 16 h light, 28 ℃/8 h dark, 43 ℃ cycle; The plants were grown in greenhouse at 16 h light, 28 ℃/8 h dark, 44 ℃ cycle; The plants were grown in greenhouse at 16 h light, 28 ℃/8 h dark, 45 ℃ cycle; The plants were grown in greenhouse at 16 h light, 28 ℃/8 h dark, 46 ℃ cycle; The plants were grown in greenhouse at 16 h light, 28 ℃/8 h dark, 47 ℃ cycle
处理方案: -
提取方案: Embryos were collected, flash frozen in liquid nitrogen, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries.
建库方案: RNA libraries were prepared for sequencing using standard Illumina protocols
测序信息
分子类型: poly(A)+ RNA
库的片段类型: PAIRED
库的链类型: Forward
测序平台: ILLUMINA
测序仪型号: Illumina HiSeq 3000
链特异性: Specific
样本
基本信息:
样本描述:
生物条件:
实验变量:
方案:
测序信息:
质量评估:
数据来源 GEN样本编号 GEN数据集编号 系列编号 项目编号 样本编号 样本名称 生物样本编号 样本访问号 实验访问号 释放时间 提交时间 最后更新时间 物种 种族 族裔 年龄 年龄单位 性别 来源名称 组织 细胞类型 细胞亚型 细胞系 疾病 疾病状态 发育阶段 突变/变异 表型 Condition Detail 生长方案 处理方案 提取方案 建库方案 分子类型 库的片段类型 链特异性 库的链类型 加标(Spike-In) 测序方法 测序平台 测序仪型号 细胞数 测序片段数 碱基数 平均测序片段长度_1 平均测序片段长度_2 唯一比对率 多重比对率 覆盖度