概要: This study evaluated transcriptomic responses to submergence in elongating and non-elongating leaves of rice near-isogenic lines with and without SUB1A using RNA-Seq. SUB1A is an ERF transcription factor gene and the key regulator of submergence tolerance in rice, restricting underwater elongation and avoiding starvation under the stress. Submergence induces mRNA accumulation of SUB1A similarly in elongating and non-elongating leaves. This study uncovered SUB1A-dependent and independent regulation of adaptive responses to submergence in the two functionally distinct leaves at the global level.
项目整体设计: mRNAs extracted from elongating and non-elongating leaves of rice plants [M202 and M202(Sub1)] submerged for 0, 1, 3, and 6 days. Three independent biological replicates.
Rice plants were grown in pots in a greenhouse until the 4.2-leaf stage.
处理方案:
Plants were completely submerged for 0, 1, 3, and 6 days. Immediately after de-submergence at each time point, the 5th (elongating) leaves and lower (non-elongating) leaves were separated, frozen in liquid nitrogen, and stored at -80C until use.
提取方案:
Total RNAs were extracted from frozen tissues using a miRNeasy Mini kit (Qiagen, Hilden, Germany). Genomic DNA was eliminated by on-column digestion.
建库方案:
RNA-Seq libraries were prepared for sequencing using standard Illumina protocols
