PRJNA758292: Postmortem high-dimensional immune profiling of severe COVID-19 patients reveals distinct patterns of immunosuppression and immunoactivation [RNA-seq]
概要: To delineate the in situ immune responses to SARS-CoV-2 viral infection, we performed comprehensive immune profiling in COVID-19 decedents. In this study, we described the immune microenvironment of COVID-19 patients through transcriptome sequencing.
项目整体设计: This dataset contains expression from SARS-CoV-2 infected patients and healthy control, various tissues.
All samples are taken from autopsy specimens, and then bulk RNA is directly extracted for sequencing
提取方案:
RNA electrophoresis was extracted from FFPE samples, 200-500bp fragments were recovered, PCR amplified and recovered fragments were constructed to construct a sequencing library.
建库方案:
The sequencing library construction and sequencing reaction used Illumina's standard procedures (Illumina, USA), and the library size 300bp, the sequencing strategy is PE150.
测序信息
分子类型:
rRNA- RNA
库的片段类型:
PAIRED
库的链类型:
Forward
测序平台:
ILLUMINA
测序仪型号:
Illumina NovaSeq 6000
链特异性:
Specific
样本
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样本描述:
生物条件:
实验变量:
方案:
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质量评估:
数据来源
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物种
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来源名称
组织
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疾病
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Condition Detail
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加标（Spike-In）
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唯一比对率
多重比对率
覆盖度
文章
Postmortem high-dimensional immune profiling of severe COVID-19 patients reveals distinct patterns of immunosuppression and immunoactivation.
