PRJNA766554: Investigation of human peripheral T cells responsive to SARS-CoV-2 antigens
概要: To elucidate the T cell epitopes of SARS-CoV-2, we stimulated human PBMCs from healthy donors and convalescent COVID-19 patients with various SARS-CoV-2 antigens, sorted the activated T cells and performed sc-RNA and -TCR sequencing. We obtained thousands of T cell clonotypes that responded to SARS-CoV-2 antigens, and identified the epitopes and restricting HLAs of several clonotypes that were significantly expanded in the COVID-19 patients.
项目整体设计: Determination of the TCR and mRNA expression of human T cells stimulated with SARS-CoV-2 antigens.
| 生长方案: |
Human PBMCs were isolated and cryo-preserved until they were thawed and used in stimulations. |
| 处理方案: |
Human PBMCs were stimulated with SARS-CoV-2 ligands and sorted before applied to sc-RNA and -TCR analysis. |
| 提取方案: |
Chromium Next GEM Single Cell 5′ Library (10x Genomics) |
| 建库方案: |
- |
| 分子类型: |
polyA(+) RNA |
| 库的片段类型: |
PAIRED |
| 库的链类型: |
- |
| 测序平台: |
ILLUMINA |
| 测序仪型号: |
Illumina NovaSeq 6000 |
| 链特异性: |
- |
| 数据来源 |
GEN样本编号 |
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样本名称 |
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最后更新时间 |
物种 |
种族 |
族裔 |
年龄 |
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性别 |
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组织 |
细胞类型 |
细胞亚型 |
细胞系 |
疾病 |
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发育阶段 |
突变/变异 |
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Condition Detail |
生长方案 |
处理方案 |
提取方案 |
建库方案 |
分子类型 |
库的片段类型 |
链特异性 |
库的链类型 |
加标（Spike-In） |
测序方法 |
测序平台 |
测序仪型号 |
细胞数 |
测序片段数 |
碱基数 |
平均测序片段长度_1 |
平均测序片段长度_2 |
唯一比对率 |
多重比对率 |
覆盖度 |
Identification of conserved SARS-CoV-2 spike epitopes that expand public cTfh clonotypes in mild COVID-19 patients.
The Journal of experimental medicine . 2021-10-14 [PMID:
34647971]
