PRJNA769125: Single Nuclei RNA Sequencing Analysis of Cardiac Microthrombi in Fatal Covid-19
概要: Cardiac microthrombi have been variably identified at autopsy of Covid-19 decedents. Little is known about the morphologic and molecular changes associated with cardiac microthrombi and whether predictive biomarkers exist. We sought to determine the prevalence, pathogenesis, and biomarker risk factors of Covid-19-associated cardiac microthrombi. In addition to histology and immunohistochemical analyses, we investigated right verticles from 8 Covid-19 decedents via single nuclei RNA sequencing for cell-type specific transcriptional differences between hearts with and without microthrombi, as well as between Covid-19 decedents and publicly available non-Covid-19 donor controls.
项目整体设计: Compare differential gene expression in right ventricles of fatal Covid-19 hearts between patients that developed microthrombi and those that did not.
| 生长方案: |
- |
| 处理方案: |
- |
| 提取方案: |
Nuclei were isolated from flash frozen post-mortem cardiac tissue for microfluidic encapsulation using 10X Genomics' 10X Genomics Chromium Controller v3.1 Next GEM Single Cell 3'. Approximately 100mg of tissue was sectioned to a thickness of 100um then subjected to differential centrifugation and filtering. |
| 建库方案: |
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| 分子类型: |
polyA(+) RNA |
| 库的片段类型: |
PAIRED |
| 库的链类型: |
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| 测序平台: |
ILLUMINA |
| 测序仪型号: |
Illumina NovaSeq 6000 |
| 链特异性: |
- |
| 数据来源 |
GEN样本编号 |
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物种 |
种族 |
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性别 |
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组织 |
细胞类型 |
细胞亚型 |
细胞系 |
疾病 |
疾病状态 |
发育阶段 |
突变/变异 |
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Condition Detail |
生长方案 |
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提取方案 |
建库方案 |
分子类型 |
库的片段类型 |
链特异性 |
库的链类型 |
加标（Spike-In） |
测序方法 |
测序平台 |
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细胞数 |
测序片段数 |
碱基数 |
平均测序片段长度_1 |
平均测序片段长度_2 |
唯一比对率 |
多重比对率 |
覆盖度 |
Clinico-histopathologic and single nuclei RNA sequencing insights into cardiac injury and microthrombi in critical COVID-19.
JCI insight . 2021-12-14 [PMID:
34905515]
