概要: Here, we investigated the function of the plant-specific SR protein RS33 in pre-mRNA splicing regulation and abiotic stress responses in rice. The loss-of-function mutant, rs33, showed increased sensitivity to salt and low-temperature stress. Genome-wide analyses of gene expression and splicing in seedlings subjected to these stresses identified multiple splice isoforms from stress-responsive genes dependent on RS33. The number of RS33-regulated genes is much higher under low-temperature stress as compared to salt stress. Our results suggest that this plant-specific splicing factor plays crucial and distinct roles during plant adaptation to abiotic stresses.
项目整体设计: 7-day-old seedlings of rs33 mutant, and WT are treated for 3hours and 6hours with 200mM NaCl, 4ºC and mock treatments.
Three hours and six hours treatment with 200mM NaCl, 4ºC and mock
提取方案:
Direct-zol RNA MiniPrep Plus (Zymo Research) according to the manufacturer’s instructions
建库方案:
The RNA-seq libraries were constructed using TruSeq mRNA stranded kit following the standard protocol and sequenced on the HiSeq-4000 platform to generate high-quality paired-end reads.
