概要: We used mRNA sequencing to study the effects of foliar piperonylic acid treatment on the transcriptome of rice plants (cv. Nipponbare) and infection by M. graminicola to gain insights in the molecular basis of piperonylic acid-induced disease resistance against this nematode
项目整体设计: mRNA sequencing of the root systems of rice seedlings treated with piperonylic acid or a mock treatment 13 days post transplantation
Rice seedlings (cv. Nipponbare) were germinated on wet tissue paper (28°C, dark), transplanted to individual PVC tubes 5 days after germination and placed in a growth chamber (28°C, 12 hours of light)
处理方案:
Plants were foliarly sprayed with a solution containing 300 µM PA and 0.1 v/v% Tween 20 or a corresponding mock solution 13 days post transplantation.
提取方案:
Plants were washed, pooled (4 plants per sample) and root and shoot tissues were ground seperately in liquid nitrogen. RNA was extracted using a Zymo Plant RNA kit as per manufacturer's instructions (Zymo Research).
建库方案:
QuantSeq 3′mRNA-Seq Library Prep Kit (Lexogen) as per manufacturer's instruction, starting from 2 µg RNA.
