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HRA000148
   HRA000148.xlsx
Title:
scCOOL-seq Reveals the Dynamic Epigenome of Human Adult Spermatogenesis
Release date:
2022-04-15
Description:
Stepwise and sequential cell fate transition accomplished by global transcriptional changes occurs across human spermatogenesis, but the epigenome roadmap and regulatory networks underlying this process have not been established. Here, we explore a single-cell multi-omics landscape of human adult testicular cells using the single-cell chromatin overall omic-scale landscape sequencing (scCOOL-seq) technique, deriving an unbiased multi-omics reference map of human adult spermatogenesis. The initial commitment to meiosis coincides with a novel dramatic DNA demethylation process in non-coding regions that highly overlapped with crossover and double strand break (DSB) hotspots. Chromatin accessibility mainly accounts for the global expression dynamics in human spermatogenesis. Further studies identify the cis- regulatory elements of several key regulators such as STRA8, ID3, HMGA1 and TEX29 in human spermatogenesis.
Data Accessibility:   
Controlled access Request Data
BioProject:
PRJCA002540
Study type:
Development and disease
Data Access Committee

For each controlled access study, there is a corresponding Data Access Committee(DAC) to determine the access permissions. Access to actual data files is not managed by NGDC.


DAC NO.:
HDAC000087
DAC name:
SMU Pathophysiology
Contact person:
Li Lin
Email:
smull22@smu.edu.cn
Description:
The individuals in SMU Pathophysiology Data Access Committee grants access to the Data. Any other individuals can request access to the Data by giving brief abstract of the Project in which the Data will be used and providing the lab information.
Individuals & samples
Files
Request Data
Submitter:   Li Lin / smull22@smu.edu.cn
Organization:   Southern Medical University
Submission date:   2020-04-15
Requests:   6