1. Home
  2. Browse
  3. HRA000216
HRA000216
   HRA000216.xlsx
Title:
Single-cell Immune Profiling of Mesenchymal Stem Cells Treatments for COVID-19
Release date:
2020-06-30
Description:
Although many efforts were spent to treat Corona Virus Disease 2019 (COVID-19), the pathogenesis and effective treatments were still absent. We performed human umbilical cord mesenchymal stem cells (hUC-MSCs) treatment for mild/moderate COVID-19 cases. Gene expression profile were detected using single-cell RNA and V(D)J sequencing. We found the functional improvement of effector memory CD4+ T cells, promotion of the anti-infective activities in effector CD8+ T cells, NK cells and plasma cells in convalescent patients after MSCs treatments. We further identified 6 high frequency T cell clonotypes. Notably, we found the global downregulation of AP-1 subunits and dysfunction of ribosomal subunits, which may affect inflammatory response and virus replication. Our results demonstrated the effects of MSCs treatments for mild/ moderate COVID-19 at single-cell resolution.
Data Accessibility:   
Controlled access Request Data
BioProject:
PRJCA002943
Study type:
Disease Study
Disease name:
COVID-19
Data Access Committee

For each controlled access study, there is a corresponding Data Access Committee(DAC) to determine the access permissions. Access to actual data files is not managed by NGDC.


DAC NO.:
HDAC000146
DAC name:
Stem Cell Therapy Committee
Contact person:
Xue Zhigang
Email:
xuezg@tongji.edu.cn
Description:
Although many efforts were spent to treat Corona Virus Disease 2019 (COVID-19), the pathogenesis and effective treatments were still absent. We performed human umbilical cord mesenchymal stem cells (hUC-MSCs) treatment for mild/moderate COVID-19 cases. Gene expression profile were detected using single-cell RNA and V(D)J sequencing. We found the functional improvement of effector memory CD4+ T cells, promotion of the anti-infective activities in effector CD8+ T cells, NK cells and plasma cells in convalescent patients after MSCs treatments. We further identified 6 high frequency T cell clonotypes. Notably, we found the global downregulation of AP-1 subunits and dysfunction of ribosomal subunits, which may affect inflammatory response and virus replication. Our results demonstrated the effects of MSCs treatments for mild/ moderate COVID-19 at single-cell resolution.
Individuals & samples
Files
Request Data
Submitter:   Xue Zhigang / xuezg@tongji.edu.cn
Organization:   Tongji University
Submission date:   2020-06-28
Requests:   1