1. Home
  2. Browse
  3. HRA000225
HRA000225
   HRA000225.xlsx
Title:
Immunoregulatory and antioxidative effects of MSCs treatment on the COVID-19-induced acute respiratory distress syndrome
Release date:
2020-07-18
Description:
Acute respiratory distress syndrome (ARDS) is the leading cause of death in severe and critical COVID-19 cases that needs effective treatments. Mesenchymal stem cells (MSCs) are effective in the treatment of various diseases, the therapeutic effects and mechanism of MSCs on COVID-19-induced ARDS need further investigation. We performed randomized trial using human umbilical cord MSCs (hUC-MSCs) for the critical COVID-19-induced and SARS-CoV-2-negative ARDS patients. We collected peripheral blood mononuclear cells from 1 treated and 1 control patient for single-cell RNA sequencing (scRNA-seq) to examine the difference in gene expression. scRNA-seq analyses revealed decrease of monocytes and restoration of T cells, as well as downregulation of the functionally related gene expression. Our study provides positive clinical evidences and possible molecular mechanisms for the immunoregulation and oxidative stress protection of MSCs treatment for COVID-19 and ARDS.
Data Accessibility:   
Controlled access Request Data
BioProject:
PRJCA002980
Study type:
Disease Study
Disease name:
COVID-19
Data Access Committee

For each controlled access study, there is a corresponding Data Access Committee(DAC) to determine the access permissions. Access to actual data files is not managed by NGDC.


DAC NO.:
HDAC000146
DAC name:
Stem Cell Therapy Committee
Contact person:
Xue Zhigang
Email:
xuezg@tongji.edu.cn
Description:
Although many efforts were spent to treat Corona Virus Disease 2019 (COVID-19), the pathogenesis and effective treatments were still absent. We performed human umbilical cord mesenchymal stem cells (hUC-MSCs) treatment for mild/moderate COVID-19 cases. Gene expression profile were detected using single-cell RNA and V(D)J sequencing. We found the functional improvement of effector memory CD4+ T cells, promotion of the anti-infective activities in effector CD8+ T cells, NK cells and plasma cells in convalescent patients after MSCs treatments. We further identified 6 high frequency T cell clonotypes. Notably, we found the global downregulation of AP-1 subunits and dysfunction of ribosomal subunits, which may affect inflammatory response and virus replication. Our results demonstrated the effects of MSCs treatments for mild/ moderate COVID-19 at single-cell resolution.
Individuals & samples
Files
Request Data
Submitter:   Xue Zhigang / xuezg@tongji.edu.cn
Organization:   Tongji University
Submission date:   2020-07-06
Requests:   -