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HRA000418
   HRA000418.xlsx
Title:
RNA-seq in the KYSE510-pCMV6/PURA cells
Release date:
2021-07-01
Description:
We performed RNA-seq in KYSE510-pCMV6 and KYSE510-PURA stable cells to identify the function of PURA. Polyadenylated mRNAs were purified and concentrated with oligo (dT)-conjugated magnetic beads. The purified mRNAs were fragmented at 95°C followed by end repair and 5' adaptor ligation and the cleaved RNA fragments were reverse-transcribed to generate cDNAs using primers containing a 3' adaptor sequence and random hexamers. The cDNAs were purified and amplified and PCR products corresponding to 200-500 bp were purified, quantified and sequenced.
Data Accessibility:   
Controlled access Request Data
BioProject:
PRJCA003814
Study type:
Cell line related study
Data Access Committee

For each controlled access study, there is a corresponding Data Access Committee(DAC) to determine the access permissions. Access to actual data files is not managed by NGDC.


DAC NO.:
HDAC000286
DAC name:
RNA-seq
Contact person:
Zhao Xiaohang
Email:
zhaoxh@cicams.ac.cn
Description:
RNA-seq in KYSE510-pCMV6/PURA cells
Individuals & samples
Files
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Submitter:   Zhao Xiaohang / zhaoxh@cicams.ac.cn
Organization:   Cancer Hospital, Chinese Academy of Medical Sciences
Submission date:   2020-11-02
Requests:   -