NGDC - GSA for Human

HRA000976

HRA000976.xlsx

Title:

Single-Cell RNA-seq Analysis of Diabetic Patients Testis Reveals Abnormal Transcriptional Signatures of Sertoli cell and Blood-Testis Barrier Dysfunction

Release date:

2022-10-28

Description:

Type 2 diabetes mellitus is one of the most prevalent metabolic diseases affecting multiple organs, including reproductive disorders in male diabetic patients. Here, we performed smart-seq2 to examine the transcriptome of diabetic patients' testis cells at single cell resolution. Intriguingly, whereas spermatogenesis appears largely preserved, the gene expression profiles of Sertoli cells and the blood-testis barrier (BTB) structure were dramatically impaired. Among the deregulated pathways, the Apelin (APLN) peptide /Apelin-receptor (APJ) axis was hyper-activated in diabetic patients. Together, these effects culminated in BTB structural dysfunction. Finally, using the small molecule APLN receptor antagonist, ML221, we show that blocking APLN/APJ significantly ameliorated the BTB damage and, importantly, improved functional spermatogenesis in diabetic db/db micse. Our findings identify the APLN/APJ axis as a promising therapeutic target to improve reproduction capacity in male diabetic patients.

Data Accessibility:

Controlled access Request Data

BioProject:

PRJCA005594

Study type:

Disease Study

Disease name:

diabetes mellitus

Data Access Committee

For each controlled access study, there is a corresponding Data Access Committee(DAC) to determine the access permissions. Access to actual data files is not managed by NGDC.

DAC NO.:

HDAC000218

DAC name:

Southern Medical University

Contact person:

Zhao Xiaoyang

Email:

zhaoxiaoyang@smu.edu.cn

Description:

We will comply with the regulations of the Data Access Committee (DAC) to determine whether to release data

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