Here we report detailed genotypes and phenotypes of TALEN-edited MECP2 mutant cynomolgus monkeys, serving as a model for a neurodevelopmental disorder, Rett syndrome (RTT), which is caused by loss of function mutations in the human MECP2 gene. Male mutant monkeys were embryonic lethal, reiterating that RTT is a disease of females. Through a battery of behavioral analyses, including primate-unique eye-tracking tests, in combination of brain imaging via MRI, we found a series of physiological, behavioral, and structural abnormalities resembling clinical manifestations of RTT. Moreover, blood transcriptome profiling revealed that mutant monkeys resembled RTT patients in immune gene dysregulation. Taken together, the stark similarity in phenotype and/or endophenotype between monkeys and patients suggested that gene-edited RTT founder monkeys would be of value for disease mechanistic studies as well as development of potential therapeutic interventions for RTT
Organization: Kunming University of Science and Technology
Submission date: 2021-08-27
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