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HRA001242
   HRA001242.xlsx
Title:
Single-cell RNA sequencing reveals regulatory mechanism for trophoblast cell-fate divergence in human peri-implantation conceptuses
Release date:
2021-09-03
Description:
Multipotent trophoblasts undergo dynamic morphological movement and cellular differentiation after conceptus implantation to generate placenta. However, the mechanism controlling trophoblast development and differentiation during peri-implantation development in human remains elusive. In this study, we modeled human conceptus peri-implantation development from blastocyst to early postimplantation stages by using an in vitro coculture system and profiled the transcriptome of 476 individual trophoblast cells from these conceptuses. We revealed the genetic networks regulating peri-implantation trophoblast development. The function of TBX3 in trophoblast differentiation is then validated by a loss-of-function experiment. In conclusion, our results provided a valuable resource to study the regulation of trophoblasts development and differentiation during human peri-implantation development.
Data Accessibility:   
Controlled access Request Data
BioProject:
PRJCA006336
Study type:
embryonic development
Data Access Committee

For each controlled access study, there is a corresponding Data Access Committee(DAC) to determine the access permissions. Access to actual data files is not managed by NGDC.


DAC NO.:
HDAC000190
DAC name:
ART Committee
Contact person:
Xue Zhigang
Email:
xuezg@tongji.edu.cn
Description:
Although many efforts were spent to treat infertility, the pathogenesis and effective treatments were still absent. For exploring the mechanism of inferility, we performed human oocyte or embyro on RNA-seq. Gene expression profile were detected using single-cell RNA sequencing.
Individuals & samples
Files
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Submitter:   Xue Zhigang / xuezg@tongji.edu.cn
Organization:   Tongji University
Submission date:   2021-08-29
Requests:   -