Generation of human hepatocytes from extended pluripotent stem cells
Release date:
2023-12-14
Description:
In this study, we sought to use human extended pluripotent stem (EPS) cells in directed differentiation by generating human hepatocytes from these cells. Our group has made great efforts in establishing directed differentiation protocols for generation of human functional hepatocytes from conventional human pluripotent stem cells. In this work, we demonstrated that human EPS cells could be efficiently differentiated into functional hepatocytes (EPS-Heps) through adding a pretreatment step before hepatic differentiation. Importantly, compared to hepatocytes derived from conventional human pluripotent stem cells, EPS-Heps transcriptionally more resemble primary human hepatocytes.
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