Glycine deprivation induces DNA damage and thereby results in cell cycle arrest in myeloma cells
Release date:
2022-05-30
Description:
Exogenous glycine deprivation inhibits tumor progression in multiple myeloma (MM) both in vitro and in vivo. To explore the underlying mechanisms of glycine metabolisms in MM cells, RNA-sequencing was performed in ARP1 cells cultured in the absence or presence of glycine (10 mg/L) for 24 hours. Genes involving glycine metabolism, among which SLC6A9, GLDC, SHMT2, and GSS were significantly highly expressed. This finding indicated that exogenous glycine deprivation mainly affected glycine transport, glycine cleavage, the interconversion between glycine and serine, and glutathione synthesis. In addition, enrichment analysis of signaling pathways based on different genes showed half of top 10 enriched pathways were related to cell mitosis and DNA damage.
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Submitter: Zhou Wen / wenzhou@csu.edu.cn
Organization: Cancer Research Institute, Central South University
Submission date: 2022-05-30
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HRA002453.xlsx