NGDC - GSA for Human

HRA002777

HRA002777.xlsx

Title:

Dynamics and signals of CD4+ T cell activation and the influencing factors revealed by single cell RNA-seq

Release date:

2023-08-17

Description:

T cell activation is a key event in adaptive immunity. However, the effects of cell status, cell heterogeneity and cellular communication on T cell activation have not been systematically analyzed. We found resting CD4 T cells differentiated into conventional effector T cells (conv TEFF), cytokines high TEFF (cytohi TEFF), proliferation TEFF (prolif TEFF) and heat shock proteins (HSPs) high expressed T (HSPhi T) upon anti-CD3/CD28 stimulation. We called the stimulated T cells overlapped with resting T on UMAP as inert T. Different from resting T, inert T showed intermediate feature of T cell activation including expression of cytokines and activated T specific marker genes. Resting CD4 T cells were more likely to differentiate into cytohi TEFF and were less likely to differentiate into HSPhi T and Th0 in the presence of CD8 T cells. Resting CD4 T cells respond to anti-CD3/CD28 differently from either CD4 TN or CD4 TM, due to difference of cell states, cell-cell communications and specific microenvironment.

Data Accessibility:

Controlled access Request Data

BioProject:

PRJCA010895

Study type:

healthy human study

Data Access Committee

For each controlled access study, there is a corresponding Data Access Committee(DAC) to determine the access permissions. Access to actual data files is not managed by NGDC.

DAC NO.:

HDAC000042

DAC name:

SUSTECH_SCPM

Contact person:

Jin Wenfei Jin

Email:

jinwf@sustech.edu.cn

Description:

SIngle-cell Sequencing in Precison Medicine

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