NGDC - GSA for Human

∗This dataset has not yet been registered in the Human Genetic Resource Management Platform of MOST

HRA003754

Title:

High-throughput single nucleus total RNA sequencing of formalin-fixed paraffin-embedded (FFPE) tissues by snRandom-seq_2

Release date:

2024-01-05

Description:

Formalin-fixed paraffin-embedded (FFPE) tissues constitute a vast and valuable patient material bank. It is still challenging to achieve single cell/nucleus RNA (sc/snRNA) profile in FFPE tissues. Here, we developed a droplet-based snRNA sequencing technology (snRandom-seq) for FFPE tissues by capturing full-length total RNAs with random primers. snRandom-seq showed a minor doublet rate (0.3%), a much higher RNA coverage, and detected more non-coding RNAs and nascent RNAs, compared with state-of-art high-throughput scRNA-seq technologies. We used snRandom-seq to profile ~20,000 single nuclei from multiple FFPE mouse tissues. A median of >3,000 genes per nucleus was detected and 25 typical cell types were identified. Moreover, we applied snRandom-seq on a clinical FFPE human liver cancer specimen and revealed an interesting subpopulation of nuclei with high proliferative activity.

Data Accessibility:

Open access

BioProject:

PRJCA014083

Study type:

Methodology

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