NGDC - GSA for Human

HRA003861

HRA003861.xlsx

Title:

Unbiased screen of cap-independent translation initiation elements reveals new complexity of translational regulation

Release date:

2023-12-31

Description:

Although most eukaryotic mRNAs require the 5'-cap for translation, some mRNAs can also be translated through a cap-independent pathway. Here we developed a cell-based system to unbiasedly screen human transcriptome for sequences that drive cap-independent circRNA translation, and identified more than 10,000 endogenous sequences. These sites were found in all mRNA regions, with features distinct from canonical IRESs, and thus were defined as Cap-independent Translation Initiation sites (CiTI). The CiTIs at 5'-UTR tend to pair with 18S rRNA to promote translation. However, the CiTIs at 3'-UTR may function either as IRESs to drive downstream ORF translation, or as translation enhancers to promote upstream main ORF translation by unwinding the highly structured 5'-UTR. We further identified trans-acting factors that specifically bind CiTIs at 3'-UTR to promote upstream ORF translation, including several components of translation initiation complexes such as DHX29.

Data Accessibility:

Controlled access Request Data

BioProject:

PRJCA014279

Study type:

Cell line related study

Data Access Committee

For each controlled access study, there is a corresponding Data Access Committee(DAC) to determine the access permissions. Access to actual data files is not managed by NGDC.

DAC NO.:

HDAC002184

DAC name:

picb_rnasys

Contact person:

wang zefeng

Email:

wangzefeng@picb.ac.cn

Description:

rnasys lab, SINH, CAS

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