To identify novel host factors that enable SARS-CoV-2 infection, we performed a genome-wide CRISPRa screen in HEK293T cells. After two rounds of infection by SARS-CoV-2 pseudovirus with an EGFP marker, the library cells were sorted for total EGFP+ cells as well as the top 10%-20%, top 10% and top 2% grouped by the EGFP intensity. The reference sample and each group of EGFP+ cells were subjected to genomic extraction, PCR amplification of the sgRNAeBAR sequences and high-throughput sequencing.
For each controlled access study, there is a corresponding Data Access Committee(DAC) to determine the access permissions. Access to actual data files is not managed by NGDC.
You need to install the Aspera Connect plugin before uploading your files. If you dont have Aspera Connect plugin installed in your computer, please Download Aspera Connectand install it first.
[path/to/ascp]: Microsoft Windows: C:\Program Files\Aspera\Aspera Connect\bin\ascp.exe or
c:\users\[username]\AppData\Local\Programs\Aspera\Aspera Connect\bin\ascp.exe
Mac OS X: /Applications/Aspera/Connect.app/Contents/Resources/ascp (for admin's installation) or
/Users/[username]/Applications/Aspera/Connect.app/Contents/Resources/ascp (for non-admin's installation)
Linux: /opt/aspera/bin/ascp or
/home/[username]/aspera/connect/bin/ascp
[path/to/key/file] must be an absolute path, e.g.: /home/keys/aspera.openssh
[path/to/folder/containing/files] needs to specify the local folder that contains all the files to upload.
HRA005140.xlsx