Here we present two optimized methods for mapping the occupancy level of a global set of TFs or certain TF of interest on native chromatin, respectively. With the binding sites highly resolved in the form of footprints protected by TF binding, we further incorporated a series of increasing perturbing forces to delineate a continuum of retention of the same TF across different binding sites, which better explains the local sequence feature and the dynamic genome organization than the occupancy level obtained from crosslinked ChIP assays.
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Individuals & samples
Files
Submitter: Xu Chenhuan / xuchh@big.ac.cn
Organization: Beijing Institute of Genomics, Chinese Academy of Sciences / China National Center for Bioinformation
Submission date: 2023-09-26
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HRA005744.xlsx