A Real-World Multi-Center RNA-seq Study Using Quartet and MAQC Reference Materials
Release date:
2023-11-08
Description:
As part of the Quartet project, we tested Quartet samples spiked with ERCC controls and MAQC samples in 45 independent laboratories, each employing its distinct RNA-seq workflow. These RNA-seq data revealed noteworthy variations in multiple aspects of the transcriptome when detecting subtle differential expression in real-world laboratory settings, especially in data quality, absolute gene expression, and differential expression calls. RNA-seq data from 13 laboratories were considered high-quality, covering several distinct experimental protocols, and used for benchmarking analysis pipelines.
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