Maternal factor OTX2 regulates human zygotic genome activation and early development
Release date:
2025-07-18
Description:
Zygotic Genome Activation (ZGA), the first transcription event in life, awakens the dormant genome and initiates the embryonic program to start the maternal-to-zygotic transition. Pre-existing maternal transcription factors (TFs) presumably are instrumental in kickstarting ZGA. How TFs regulate human ZGA in early embryos remains largely unknown. OTX2, a maternal PRD-like homeobox domain TF, is highly activated for its translation prior to human ZGA. By depleting OTX2 in human embryos, we found OTX2 is required for proper minor ZGA and early human development. This is achieved in part by binding and opening chromatin near ZGA genes. Finally, joint knockdown of OTX2 and TPRXL, the two major maternal PRD-like homeobox TFs, exacerbates inefficient chromatin opening, major ZGA defects and results in severe developmental abnormalities. These findings underscore a crucial role of maternal TFs in regulating human ZGA and early development.
Note: HTTP download speed may be slow. It is highly recommended that you download the dataset using a dedicated FTP tool (such as FileZilla Client) or Asepra Command Line.
Individuals & samples
Files
Submitter: Xie Wei / xiewei121@tsinghua.edu.cn
Organization: Tsinghua University
Submission date: 2024-01-31
Aspera Command Line
You need to install the Aspera Connect plugin before uploading your files. If you dont have Aspera Connect plugin installed in your computer, please Download Aspera Connectand install it first.
[path/to/ascp]: Microsoft Windows: C:\Program Files\Aspera\Aspera Connect\bin\ascp.exe or
c:\users\[username]\AppData\Local\Programs\Aspera\Aspera Connect\bin\ascp.exe
Mac OS X: /Applications/Aspera/Connect.app/Contents/Resources/ascp (for admin's installation) or
/Users/[username]/Applications/Aspera/Connect.app/Contents/Resources/ascp (for non-admin's installation)
Linux: /opt/aspera/bin/ascp or
/home/[username]/aspera/connect/bin/ascp
[path/to/key/file] must be an absolute path, e.g.: /home/keys/aspera.openssh
[path/to/folder/containing/files] needs to specify the local folder that contains all the files to upload.
Note: HTTP download speed may be slow. It is highly recommended that you download the dataset using a dedicated FTP tool (such as FileZilla Client) or Asepra Command Line.
HRA006621.xlsx