CRISPR-Cas9 Knockout Screen Identifies factors involved in the cytotoxicity of Decitabine
Release date:
2025-01-10
Description:
Decitabine (DAC), a first-line drug for acute myeloid leukemia (AML), has been long-recognized for its DNA demethylation effects. However, its full spectrum of anti-tumor mechanisms remains incompletely understood. Here, we employ a temporally resolved genome-wide CRISPR-Cas9 screen to identify key factors governing DAC response. We also use lncRNA-seq and ChIP-seq techiques to investigate the changes of trancriptome and chromatin occupancy of proteins of interest.
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Organization: Institute of Biophysics, Chinese Academy of Sciences
Submission date: 2024-02-18
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HRA006701.xlsx