RNA-seq of negative control and KRT32-overexpressing Ker-CT cells
Release date:
2024-03-18
Description:
The RNA-seq experiment involved three replicate samples of negative control and KRT32-overexpressing Ker-CT cells. The cells were initially cultured for 24 hours in a 12-well plate with a cell count of 10^6. Total RNA was extracted using the RNAiso Plus kit (TAKARA, Japa). Eukaryotic mRNA enrichment was achieved using Oligo(dT) beads, and the enriched mRNA fragments were further fragmented using fragmentation buffer. These fragments were then reverse transcribed into cDNA using the NEBNext Ultra RNA Library Prep Kit for Illumina from New England Biolabs. After end-repair and addition of A bases to the double-stranded cDNA fragments, Illumina sequencing adapters were ligated. The ligated fragments underwent size selection via agarose gel electrophoresis and were then amplified through PCR. Finally, the resulting cDNA library was sequenced on an Illumina Novaseq6000 platform.
Note: HTTP download speed may be slow. It is highly recommended that you download the dataset using a dedicated FTP tool (such as FileZilla Client) or Asepra Command Line.
Organization: Shandong Provincial Hospital for Skin Diseases & Shandong Provincial Institute of Dermatology and Veneorology, Shandong First Medical University & Shandong Academy of Medical Sciences
Submission date: 2024-03-18
Aspera Command Line
You need to install the Aspera Connect plugin before uploading your files. If you dont have Aspera Connect plugin installed in your computer, please Download Aspera Connectand install it first.
[path/to/ascp]: Microsoft Windows: C:\Program Files\Aspera\Aspera Connect\bin\ascp.exe or
c:\users\[username]\AppData\Local\Programs\Aspera\Aspera Connect\bin\ascp.exe
Mac OS X: /Applications/Aspera/Connect.app/Contents/Resources/ascp (for admin's installation) or
/Users/[username]/Applications/Aspera/Connect.app/Contents/Resources/ascp (for non-admin's installation)
Linux: /opt/aspera/bin/ascp or
/home/[username]/aspera/connect/bin/ascp
[path/to/key/file] must be an absolute path, e.g.: /home/keys/aspera.openssh
[path/to/folder/containing/files] needs to specify the local folder that contains all the files to upload.
Note: HTTP download speed may be slow. It is highly recommended that you download the dataset using a dedicated FTP tool (such as FileZilla Client) or Asepra Command Line.
HRA006924.xlsx