PBMC were isolated via Lymphocyte Separation Media (Multi Science, 70-LSM01) following the protocol. Cells were washed twice using cold PBS and total RNA was extracted with the RNeasy micro kit (QIAGEN) according to the manual. RNA sequencing was performed with a Truseq RNA library prep kit v2 (Illumina) and illumina NovaSeq 6000 equipment. We used a perlscript to filter out low quality reads and subsequently the clean reads were mapped to human genome hg19 with TopHat2. Gene expression levels were calculated and normalized to fragments per kilobase of transcript per million mapped reads (FPKM).
Organization: Life Sciences Institute, Zhejiang University
Submission date: 2024-04-20
Aspera Command Line
You need to install the Aspera Connect plugin before uploading your files. If you dont have Aspera Connect plugin installed in your computer, please Download Aspera Connectand install it first.
[path/to/ascp]: Microsoft Windows: C:\Program Files\Aspera\Aspera Connect\bin\ascp.exe or
c:\users\[username]\AppData\Local\Programs\Aspera\Aspera Connect\bin\ascp.exe
Mac OS X: /Applications/Aspera/Connect.app/Contents/Resources/ascp (for admin's installation) or
/Users/[username]/Applications/Aspera/Connect.app/Contents/Resources/ascp (for non-admin's installation)
Linux: /opt/aspera/bin/ascp or
/home/[username]/aspera/connect/bin/ascp
[path/to/key/file] must be an absolute path, e.g.: /home/keys/aspera.openssh
[path/to/folder/containing/files] needs to specify the local folder that contains all the files to upload.
