Stereo-seq V2: spatial mapping of total RNA on FFPE sections with high resolution
Release date:
2025-09-10
Description:
Performing total RNA profiling on FFPE samples, which are the predominate sample conservation method in clinical practice, is a big challenge for current spatial transcriptomics techniques. Here we brought Stereo-seq V2, an upgraded spatial transcriptomics technique that uses random primers to capture and sequence RNAs in situ on FFPE sections. We tested Stereo-seq V2 on FFPE samples of different RNA quality, and observed a robust tolerance to RNA degradation even on a sample with DV200 = 18.
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HRA007387.xlsx