Investigation of the pro-myocardial injury repair effect of Y27632/CHIR99021/SB431542 based on human engineered heart tissue
Release date:
2024-07-03
Description:
In this project, we developed a myocardial injury repair model that can mimic myocardial pathological remodeling process from cellular stress to fibrotic scar formation that triggers electrophysiological abnormality after myocardial injury in vitro by introducing cryoinjury into human engineered heart tissues (hEHT). Based on this hEHT injury repair model, this project further explored the roles of ROCK signaling inhibitor Y27632, WNT signaling activator CHIR99021, and TGFb signaling inhibitor SB431542 in the regulation of apoptosis, inflammation, and fibrosis through bulk RNA sequencing toward hEHT, aiming to elucidate the roles of the above targets in the repair of myocardial injury.
Note: HTTP download speed may be slow. It is highly recommended that you download the dataset using a dedicated FTP tool (such as FileZilla Client) or Asepra Command Line.
You need to install the Aspera Connect plugin before uploading your files. If you dont have Aspera Connect plugin installed in your computer, please Download Aspera Connectand install it first.
[path/to/ascp]: Microsoft Windows: C:\Program Files\Aspera\Aspera Connect\bin\ascp.exe or
c:\users\[username]\AppData\Local\Programs\Aspera\Aspera Connect\bin\ascp.exe
Mac OS X: /Applications/Aspera/Connect.app/Contents/Resources/ascp (for admin's installation) or
/Users/[username]/Applications/Aspera/Connect.app/Contents/Resources/ascp (for non-admin's installation)
Linux: /opt/aspera/bin/ascp or
/home/[username]/aspera/connect/bin/ascp
[path/to/key/file] must be an absolute path, e.g.: /home/keys/aspera.openssh
[path/to/folder/containing/files] needs to specify the local folder that contains all the files to upload.
Note: HTTP download speed may be slow. It is highly recommended that you download the dataset using a dedicated FTP tool (such as FileZilla Client) or Asepra Command Line.
HRA007850.xlsx