NGDC - GSA for Human

HRA008624

HRA008624.xlsx

Title:

High-resolution, noninvasive single-cell lineage tracing in mice and humans based on DNA methylation epimutations

Release date:

2024-11-16

Description:

In vivo lineage tracing holds great potential to reveal fundamental principles of tissue development and homeostasis. However, lineage tracing in humans relies on DNA mutations that are extremely rare. In mice, the improved genetic labeling approach has low resolution over cell division histories. Here, we demonstrated for the first time that frequent epimutations on DNA methylation can be exploited to infer lineage histories in normal cells, enabled by our newly developed computational method MethyTree. Using both in-house and public sparse single-cell DNA methylation datasets with known lineage labels, MethyTree reconstructed lineage histories at high resolution and accuracy across different cell types, stages, and species. Applying MethyTree, we identified the first fate decision in human embryo development and pinpointed in total ~230 clones of hematopoietic stem cells in mice. Our study opens the door for high-resolution, noninvasive lineage tracing in mice, humans and beyond.

Data Accessibility:

Controlled access Request Data

BioProject:

PRJCA030310

Study type:

Lineage tracing

Data Access Committee

For each controlled access study, there is a corresponding Data Access Committee(DAC) to determine the access permissions. Access to actual data files is not managed by NGDC.

DAC NO.:

HDAC004737

DAC name:

Westlake_WangLab

Contact person:

WANG SHOUWEN

Email:

wangshouwen@westlake.edu.cn

Description:

Laboratory of Computational Genomics and Lineage Tracing

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