| Accession |
HRX035741 |
| title |
Qihe2 |
| BioProject |
PRJCA002264 |
| Study accession |
HRA000123 |
| Individual |
HRI029480 |
| Sample |
HRS029480 |
| Platform |
Illumina HiSeq 4000 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection
| Layout |
|
DNA was extracted from less than 100 mg of bone or tooth powder from samples deriving from 26 ancient human remains. For all but six specimens, a single-stranded protocol (“SS”) was used to prepare the libraries. The single-stranded libraries for Boshan, Liangdao1 and Liangdao2 were treated with uracil-DNA-glycosylase (UDG) from E. coli and endonuclease (Endo VIII) (“SS UDG”). Libraries for Yumin, Xiaojingshan and Xiaogao were prepared using a double-stranded protocol (“DS”), while libraries for Bianbian were prepared using both single-stranded and double-stranded protocols. Libraries were amplified using the AccuPrimepfx DNA enzyme. The amplification process underwent 35 cycles to make sure enough ancient DNA was available for capture. To specific libraries, the P5 and P7 primers were added, and a NanoDrop2000 spectrometer was used to determine the amount of DNA extracted per sample. |
WGS |
GENOMIC |
PCR |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 62
|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| HRR051942 |
Qihe2_d.bam
|
33.17
|
|
|
Release date
|
2020-05-13
|
| Submitter |
Qiaomei Fu (fuqiaomei@ivpp.ac.cn)
|
| Organization |
Institute of Vertebrate Paleontology and Paleoanthropology, Chinese Academy of Sciences |
| Submission date |
2020-03-11 |
