| Accession |
HRX1121626 |
| title |
Whole genome sequencing38 |
| BioProject |
PRJCA021101 |
| Study accession |
HRA005970 |
| Individual |
HRI753560 |
| Sample |
HRS989094 |
| Platform |
BGISEQ-500 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection
| Layout |
|
High molecular weight genomic DNA from frozen tissues or whole blood samples was isolated using the QIAamp DNA Mini Kit according to the manufacturer’s protocol.1 μg genomic DNA was randomly fragmented by Covaris to an average size of 200-400bp (WGS). Fragmented DNAs were tested by agarose gel electrophoresis and purified with the AxyPrep Mag PCR clean up kit. The selected fragments were subjected to end-repair, 3’ adenylated, adapter ligation, PCR amplifying and the PCR products were recovered using the AxyPrep Mag PCR clean up Kit. For WGS, the double stranded PCR products were heat denatured and circularized by the splint oligo sequence. Single strand circle DNA (ssCir DNA) was formatted as the final library and qualified by an Agilent Technologies 2100 bioanalyzer and ABI StepOnePlus Real-Time PCR System. The qualified libraries were sent for 2 × 100-bp paired-end sequencing on the BGISEQ-500 platform. |
WGS |
GENOMIC |
PCR |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 100
Planned read length (bp) for mate 2: 100
|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| HRR1443236 |
190317_I111_CL100118693_L2_HUMtfbRAAAO-524_1.clean.fq.gz
190317_I111_CL100118693_L2_HUMtfbRAAAO-524_2.clean.fq.gz
|
7,846.88
8,044.05
|
| HRR1443235 |
190317_I111_CL100118693_L2_HUMtfbRAAAO-523_1.clean.fq.gz
190317_I111_CL100118693_L2_HUMtfbRAAAO-523_2.clean.fq.gz
|
6,962.33
7,129.03
|
| HRR1443234 |
190317_I111_CL100118693_L2_HUMtfbRAAAO-522_1.clean.fq.gz
190317_I111_CL100118693_L2_HUMtfbRAAAO-522_2.clean.fq.gz
|
7,357.77
7,462.16
|
| HRR1443233 |
190317_I111_CL100118693_L2_HUMtfbRAAAO-521_1.clean.fq.gz
190317_I111_CL100118693_L2_HUMtfbRAAAO-521_2.clean.fq.gz
|
6,468.68
6,590.35
|
| HRR1443232 |
190317_I111_CL100118693_L2_HUMtfbRAAAO-520_1.clean.fq.gz
190317_I111_CL100118693_L2_HUMtfbRAAAO-520_2.clean.fq.gz
|
7,045.99
7,283.25
|
| HRR1443231 |
190317_I111_CL100118693_L2_HUMtfbRAAAO-519_1.clean.fq.gz
190317_I111_CL100118693_L2_HUMtfbRAAAO-519_2.clean.fq.gz
|
7,672.7
7,799.21
|
| HRR1443230 |
190317_I111_CL100118693_L2_HUMtfbRAAAO-518_1.clean.fq.gz
190317_I111_CL100118693_L2_HUMtfbRAAAO-518_2.clean.fq.gz
|
7,439.38
7,510.34
|
| HRR1443229 |
190317_I111_CL100118693_L2_HUMtfbRAAAO-517_1.clean.fq.gz
190317_I111_CL100118693_L2_HUMtfbRAAAO-517_2.clean.fq.gz
|
8,212.33
8,365.45
|
|
|
Release date
|
2024-09-13
|
| Submitter |
Yuexiang Wang (yxwang76@sinh.ac.cn)
|
| Organization |
Shanghai Institute of Nutrition and Health, Chinese Academy of Sciences |
| Submission date |
2023-11-08 |
