| Accession |
HRX1121733 |
| title |
Exome107 |
| BioProject |
PRJCA021101 |
| Study accession |
HRA005970 |
| Individual |
HRI753546 |
| Sample |
HRS989073 |
| Platform |
Illumina HiSeq X Ten |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection
| Layout |
|
High molecular weight genomic DNA from frozen tissues or whole blood samples was isolated using the QIAamp DNA Mini Kit according to the manufacturer’s protocol.1 μg genomic DNA was randomly fragmented by Covaris to an average size of 250-300bp (WES). Fragmented DNAs were tested by agarose gel electrophoresis and purified with the AxyPrep Mag PCR clean up kit. The selected fragments were subjected to end-repair, 3’ adenylated, adapter ligation, PCR amplifying and the PCR products were recovered using the AxyPrep Mag PCR clean up Kit. For WES, libraries were constructed using an Agilent SureSelect Human All Exon V6 kit, followed by 2 × 150-bp paired-end sequencing on the Illumina HiSeq X10 platform. |
WES |
GENOMIC |
PCR |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| HRR1442835 |
FCHFLJNCCXY_L4_HUMmbbEAAAFAAA-306_1.fq.gz
FCHFLJNCCXY_L4_HUMmbbEAAAFAAA-306_2.fq.gz
|
5,481.72
6,515.37
|
|
|
Release date
|
2024-09-13
|
| Submitter |
Yuexiang Wang (yxwang76@sinh.ac.cn)
|
| Organization |
Shanghai Institute of Nutrition and Health, Chinese Academy of Sciences |
| Submission date |
2023-11-08 |
