| Accession |
HRX1202315 |
| title |
PT1 lvpw TCR-seq |
| BioProject |
PRJCA023994 |
| Study accession |
HRA006808 |
| Individual |
HRI806778 |
| Sample |
HRS1062470 |
| Platform |
Illumina NovaSeq 6000 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection
| Layout |
|
Total RNA was isolated from the thawed mononuclear cells from the heart using the RNeasy Plus Mini Kit (Qiagen). RNA samples were analyzed by NGS for TRBs using the ImmuHub® TCR profiling system . Briefly, a 5’ RACE unbiased amplification protocol was used. One common forward adaptor primer and one reverse primer corresponding to the constant (C) regions of each of the TCR β were designed to facilitate PCR amplification of cDNA sequences in a less biased manner. This protocol uses unique molecular identifiers (UMIs) introduced in the course of cDNA synthesis to control bottlenecks and to eliminate PCR and sequencing errors. Sequencing was performed on an Illumina NovaSeq® system. |
TCR-Seq |
TRANSCRIPTOMIC |
RACE |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| HRR1534599 |
23-11-00013AB_R1.fastq.gz
23-11-00013AB_R2.fastq.gz
|
376.77
364.48
|
|
|
Release date
|
2026-03-04
|
| Submitter |
Jian'an Wang (wang_jian_an@vip.tom.com)
|
| Organization |
The Second Affiliated Hospital, Zhejiang University School of Medicine |
| Submission date |
2024-03-04 |
