| Accession |
HRX2381613 |
| title |
HEK293T transfected with ZCWPW2-Flag |
| BioProject |
PRJCA052157 |
| Study accession |
HRA015008 |
| Individual |
HRI1658583 |
| Sample |
HRS2144477 |
| Platform |
Illumina NovaSeq 6000 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection
| Layout |
|
Cleavage Under Targets and Tagmentation (CUT&Tag) libraries were prepared following the standard protocol. HEK293T cells transfected with Flag-ZCWPW2 alone or co-transfected with Flag-ZCWPW2 and Myc-PRDM9 were bound to ConA magnetic beads and incubated with primary anti-Flag antibody or IgG control overnight. After secondary antibody incubation, cells were treated with pA-Tn5 for targeted tagmentation. DNA fragments were purified using AMPure XP beads and amplified by PCR. Libraries were sequenced on an Illumina platform. |
ChIP-Seq |
GENOMIC |
PCR |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| HRR2903189 |
ZCWPW2.R1.fastq.gz
ZCWPW2.R2.fastq.gz
|
1,659.72
1,523.37
|
|
|
Release date
|
2026-01-06
|
| Submitter |
Ying Shen (yingcaishen01@163.com)
|
| Organization |
West China The Second Hospital |
| Submission date |
2025-11-28 |
