| Accession | CRX031498 |
| Organism | Erysipelothrix rhusiopathiae |
| Title | G4T10-1 |
| BioProject | PRJCA000447 |
| BioSample | SAMC047400 |
| Platform | Illumina HiSeq 2000 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Total RNA of strain G4T10 and SE38 was extracted by means of Trizol method. For ribosomal RNAs deletion, epicentreâs Ribo-Zero⢠rRNA Removal Kits (Gram-Negative Bacteria) was used following the manufacturerâs instructions. We constructed strand-specific cDNA libraries (two biological replicates for each strain) with KAPA Stranded mRNA-Seq Kit Illumina® platform(KAPA BIOSYSTEMS, dUTP Based) according to the manufacturerâs instructions. Paired-end libraries with insert sizes of 300-400 bp, for strain G4T10 and SE38 were sequenced using Illumina HiSeq2000 with a sequencing read length of 101 bp. |
RNA-Seq |
TRANSCRIPTOMIC |
other |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 101
Planned read length (bp) for mate 2: 101
Insert size (bp): 400
|
| Release date | 2020-10-19 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR035369 |
CRR035369_f1.fastq.gz
CRR035369_r2.fastq.gz
|
982.14
977.33
|
|
| Submitter | Zhewen Zhang (zhangzw@big.ac.cn) |
|---|
| Organization | Beijing Institute of Genomics, Chinese Academy of Sciences |
|---|
| Date submitted | 2018-10-23 |
|---|
