| Accession | CRX020252 |
| Organism | Saccharomyces cerevisiae |
| Title | MNase-Seq 5min |
| BioProject | PRJCA000622 |
| BioSample | SAMC017118 |
| Platform | Illumina HiSeq 2500 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
| MNase-Seq 5min |
We adjusted the NP-S buffer and used 100 units of MNase to digest nuclei for 5 minutes. After bound nucleosomes digested with Protease K, we exacted core particle DNA. Paired-end libraries were constructed using Illumina compatible DNA-Seq NGS library preparation kit from Gnomegen and were sequenced with Illumina HiSeq 2500 (PE125, paired-end 125 bp). |
MNase-Seq |
GENOMIC |
MNase |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 125
Planned read length (bp) for mate 2: 125
Insert size (bp): 240
|
| Release date | 2018-04-19 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR022401 |
CRR022401_f1.fastq.gz
CRR022401_r2.fastq.gz
|
382.83
369.46
|
|
| Submitter | Qing Huan (qhuan@genetics.ac.cn) |
|---|
| Organization | Institute of Genetics and Developmental Biology, Chinese Academy of Sciences |
|---|
| Date submitted | 2017-11-06 |
|---|
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