Experiment information
Accession CRX020536
Organism Mus
Title PDGF induced CM proliferation and regeneration
BioProject PRJCA000636
BioSample SAMC017386
Platform Illumina HiSeq X Ten
Library name Construction protocol Strategy Source Selection Layout
NULL-3 CMs were isolated from P1 neonatal hearts of PdgfrbD849Vfl/fl mice by using the neonatal dissociation kit and isolation kit (gentleMACS) .For the virus infection experiments, CMs were infected with 300 MOI of Ad-CMV-Cre or Ad-CMV-Null (Hanbio, ShangHai, China) in DMEM/F12 medium.48 hours later, CMs were harvested for RNA-seq RNA-Seq TRANSCRIPTOMIC cDNA PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 300
Release date2017-11-19
Run accession Release date Run data file information
File nameFile size (MB)
CRR022672 2017-11-19 GFP3_R1.fastq.gz
SubmitterYUE Zhang (yuegaoxin1120@163.com)
OrganizationFuwai Hospital, Chinese Academy of Medical Sciences
Date submitted2017-11-16
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