Experiment information
Accession CRX021673
Organism Homo sapiens
Title ILMN21T
BioProject PRJCA000610
BioSample SAMC018548
Platform Illumina HiSeq 2000
Library name Construction protocol Strategy Source Selection Layout
Human genomic DNA was extracted from frozen tumor or matched colon tissue with QIAampDNA Mini kits (Qiagen). Covaris system is used to obtain the fragmented genomic DNA between 150 to 200bp. Adaptors were ligated to both ends of the fragments and adaptor-ligated templates were then purified using AgencourtAMPure SPRI beads. Whole-exome enrichment was performed by the SureSelect Human All Exon 51M kit (Agilent). Captured DNA libraries are sequenced by HiSeq 2000 Genome Analyzer (Illumina). WXS GENOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 90
Planned read length (bp) for mate 2: 90
Insert size (bp): 170
Release date2018-05-29
Run accession Release date Run data file information
File nameFile size (MB)
CRR023917 2018-05-29 ILMN21T.bam 11,943.3
SubmitterZhe Liu (liuzhebiosci@163.com)
OrganizationAnzhen Hospital
Date submitted2017-12-01
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