| 实验编号 | CRX021771 |
| 物种名称 | Homo sapiens |
| 标题 | ILMN21N |
| 项目编号 | PRJCA000610 |
| 样本编号 | SAMC018646 |
| 测序平台 | Illumina HiSeq 2000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
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Human genomic DNA was extracted from frozen tumor or matched colon tissue with QIAampDNA Mini kits (Qiagen). Covaris system is used to obtain the fragmented genomic DNA between 150 to 200bp. Adaptors were ligated to both ends of the fragments and adaptor-ligated templates were then purified using AgencourtAMPure SPRI beads. Whole-exome enrichment was performed by the SureSelect Human All Exon 51M kit (Agilent). Captured DNA libraries are sequenced by HiSeq 2000 Genome Analyzer (Illumina). |
WXS |
GENOMIC |
PCR |
PAIRED
|
|
| 处理信息 |
Planned read length (bp) for mate 1: 90
Planned read length (bp) for mate 2: 90
Insert size (bp): 170
|
| 发布日期 | 2018-05-29 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR024015 |
CRR024015.bam
|
13,571.51
|
|
| 提交者 | Zhe Liu (liuzhebiosci@163.com) |
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| 所属单位 | Anzhen Hospital |
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| 提交日期 | 2017-12-01 |
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