| 实验编号 | CRX028842 |
| 物种名称 | Ophiocordyceps sinensis |
| 标题 | A comparative trancriptomic- characterization of naturally- and artificially-cultivated Ophiocordyceps sinensis |
| 项目编号 | PRJCA000970 |
| 样本编号 | SAMC044636 |
| 测序平台 | Illumina HiSeq 2000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
| cDNA |
RNA was extracted from the fruiting body of the natural-growth O. sinensis, purified using magnetic beads with oligo (dT), and then interrupted into the fragments of about 200-700 nt in fragmentation buffer, which were used as templates to synthesize the first-strand cDNA using reverse transcriptase and random hexamer-primers. The constructed cDNA libraries were constructed through A-tailing, adaptor ligation and sequenced with the Illumina HiSeq 2000 using the paired-end 125bp method.
|
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
|
|
| 处理信息 |
Planned read length (bp) for mate 1: 100
Planned read length (bp) for mate 2: 100
Insert size (bp): 200
Planned read length (bp): 125
|
| 发布日期 | 2019-04-02 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR032207 |
CRR032207_f1.fastq.gz
CRR032207_r2.fastq.gz
|
3,033.93
3,759.91
|
|
| 提交者 | tong xinxin (1842161947@qq.com) |
|---|
| 所属单位 | Beijing Institute of Genomics, Chinese Academy of Sciences |
|---|
| 提交日期 | 2018-08-12 |
|---|
