| Accession | CRX032030 |
| Organism | Rattus norvegicus |
| Title | Diabetes 5 |
| BioProject | PRJCA001099 |
| BioSample | SAMC047713 |
| Platform | Illumina HiSeq X Ten |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Libraries for sequencing were constructed with NEB Next Ultra RNA Library Prep Kit for Illumina (NEB). Poly(A) tailed mRNA molecules were enriched from 5 ?g total RNA with NEB Next Poly(A) mRNA Magnetic Isolation Module (NEB) kit.The mRNA was fragmented into approximately 200 base pair pieces. The first-strand cDNA was synthesized from the mRNA fragments reverse transcriptase and random hexamer primers, and then the second-strand cDNA was synthesized using DNA polymerase I and RNaseH. The end of the cDNA fragment was subjected to an end repair process that included the addition of a single ‘A’ base, followed by ligation of the adapters. Products were purified and enriched by polymerase chain reaction (PCR) to amplify the library DNA. The final libraries were quantified using KAPA Library Quantification Kit (KAPA Biosystems, South Africa) and an Agilent 2100 Bioanalyzer. After quantitative reverse transcription-polymerase chain reaction (RT-qPCR) validation, libraries were subjected to paired-end sequencing with 150-base pair read length on the Illumina HiSeq X ten sequencer. |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 200
|
| Release date | 2018-11-01 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR035910 |
CRR035910_f1.fq.gz
CRR035910_r2.fq.gz
|
1,958.72
2,115.22
|
|
| Submitter | Xizhan Xu (xuxz@im.ac.cn) |
|---|
| Organization | Institute of Microbiology, Chinese Academy of Sciences |
|---|
| Date submitted | 2018-10-31 |
|---|
