| 实验编号 | CRX032696 |
| 物种名称 | Bacteria |
| 标题 | JM.21.1 for bacteria study |
| 项目编号 | PRJCA001121 |
| 样本编号 | SAMC048239 |
| 测序平台 | Illumina HiSeq 2500 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
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Total genomic DNA was extracted from soil samples using the MP FastDNA SPIN Kit for soil (MP Biomedicals, Solon, OH, USA) as per the manufacturer’s instructions. Bacterial 16S rRNA gene V4?V5 hypervariable regions were PCR-amplified using primers 515F (5’-GTG CCA GCM GCC GCG GTA A-3’) and 907R (5’-CCG TCA ATT CCT TTG AGT TT-3’) combined with adapter sequences and barcode sequences. |
AMPLICON |
METAGENOMIC |
PCR |
SINGLE
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|
| 处理信息 |
Planned read length (bp): 373
|
| 发布日期 | 2019-03-08 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR036875 |
CRR036875.fastq.gz
|
10.75
|
|
| 提交者 | Shuo Jiao (js1990@nwsuaf.edu.cn) |
|---|
| 所属单位 | Northwest A&F University |
|---|
| 提交日期 | 2018-11-19 |
|---|
