At the V9 stage, total RNA was extracted from the tissue of the 11th leaf, collected from the pool of three plants of similar growth status per line, following the standard protocol of Quick RNA isolation Kit, #0416-50. A library with insert sizes ranging from 200~500 bp was prepared using the commercial Illumina library preparation kits (TruSeq Stranded mRNA LT-SetA. RS-122-1201).
RNA-Seq
TRANSCRIPTOMIC
PolyA
PAIRED
Processing
Planned read length (bp) for mate 1: 150 Planned read length (bp) for mate 2: 150 Insert size (bp): 350
