Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
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Total RNA was extracted using CTAB-LiCl method,and genomic DNA contamination was removed using DNase I (TaKaRa, Dalian, China).RNA quality was verified by agarose gel electrophoresis and a Bioanalyzer 2100 (Agilent,CA, USA). For cDNA library construction, mRNA was enriched with oligo (dT) magnetic beads and then broken into smaller pieces using fragmentation buffer. The first strand cDNA was reversed-transcribed by random hexamers and small fragment as templates.This was followed by second strand cDNA synthesis using DNA Polymerase I and RNase H. The double strand cDNA was ligated to paired-end adapters, and suitable fragments were selected and enriched with PCR amplification. cDNA libraries were sequenced performed using an Illumina HiSeq2000 platform. |
RNA-Seq |
TRANSCRIPTOMIC |
unspecified |
PAIRED
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