| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
DNA of sorted -80? refrigera was extracted from human fecal using a qiagen DNA powersoil kit,shear NA of sorted NCSCs was extracted from the cell line using a QIAamp DNA Mini Kit, sheared to approximately 300-500 bp using a Covaris S220 instrument. Then the libraries were constructed through end-repair, A-tailing, adapter ligation and bisulfate-converted using a ZymoEZ DNA Methylation Kit. Then it was sequenced on Thermo Fisher Scientific Ion S5 XL platform. |
AMPLICON |
METAGENOMIC |
PCR |
SINGLE
|
|
