Accession | CRX048567 |
Organism | Ovis aries |
Title | we sequence this Minxian Black Fur sheep MXS9 with whole-genome resequencing |
BioProject | PRJCA001227 |
BioSample | SAMC067927 |
Platform | Illumina HiSeq 2000 |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Genomic DNA was extracted from ear tissue using the standard phenol-chloroform protocol. High-quality DNA for genome sequencing was processed to construct short-insert (500 bp) DNA libraries according to the manufacturer’s specifications. To generate 500-bp mate-paired libraries, we used the Covaris Ultrasonic Processor to cut genomic DNA into 500-bp fragments randomly, followed by the process of end repairing, adding A to the tails, purification and PCR amplification. The qualified libraries with appropriate insert size (500 bp) and concentration (> 2 nM) were sequenced using the Illumina HiSeq 2000 platform, and 100-bp paired-end reads were generated and managed using Illumina HiSeq Control Software (HCS) v3.3. |
WGS |
GENOMIC |
PCR |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 100
Planned read length (bp) for mate 2: 100
Insert size (bp): 500
|
Release date | 2019-05-27 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR053750 |
CRR053750.bam
|
12,104.23
|
|
Submitter | ? ?? (1337657902@qq.com) |
Organization | Institute of Zoology, Chinese Academy of Sciences |
Date submitted | 2019-04-19 |